Synthesis of Glutathione in Leaves of Transgenic Poplar Overexpressing [gamma]-Glutamylcysteine Synthetase.
Identifieur interne : 004A29 ( Main/Exploration ); précédent : 004A28; suivant : 004A30Synthesis of Glutathione in Leaves of Transgenic Poplar Overexpressing [gamma]-Glutamylcysteine Synthetase.
Auteurs : G. Noctor [France] ; M. Strohm ; L. Jouanin ; K. J. Kunert ; C. H. Foyer ; H. RennenbergSource :
- Plant physiology [ 1532-2548 ] ; 1996.
Abstract
Internode stem fragments of the poplar hybrid Populus tremula x Populus alba were transformed with a bacterial gene (gshl) for [gamma]-glutamylcysteine synthetase ([gamma]-ECS) targeted to the cytosol. Lines overexpressing [gamma]-ECS were identified by northern analysis, and the transformant with the highest enzyme activity was used to investigate the control of glutathione synthesis. Whereas foliar [gamma]-ECS activity was below the limit of detection in untransformed plants, activities of up to 8.7 nmol mg-1 protein min-1 were found in the transformant, in which the foliar contents of [gamma]-glutamylcysteine ([gamma]-EC) and glutathione were increased approximately 10- and 3-fold, respectively, without affecting either the reduction state of the glutathione pool or the foliar cysteine content. A supply of exogenous cysteine to leaf discs increased the glutathione content from both transformed and untransformed poplars, and caused the [gamma]-EC content of the transformant discs to increase still further. The following conclusions are drawn: (a) the native [gamma]-ECS of untransformed poplars exists in quantities that are limiting for foliar glutathione synthesis; (b) foliar glutathione synthesis in untransformed poplars is limited by cysteine availability; (c) in the transformant interactions between glutathione synthesis and cysteine synthesis operate to sustain the increased formation of [gamma]-EC and glutathione; and (d) the foliar glutathione content of the transformant is restricted by cysteine availability and by the activity of glutathione synthetase.
DOI: 10.1104/pp.112.3.1071
PubMed: 12226433
PubMed Central: PMC158033
Affiliations:
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Noctor</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire du Metabolisme (G.N.) and Laboratoire de Biologie Cellulaire (L.J.), Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire du Metabolisme (G.N.) and Laboratoire de Biologie Cellulaire (L.J.), Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Strohm, M" sort="Strohm, M" uniqKey="Strohm M" first="M." last="Strohm">M. Strohm</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name></author><author><name sortKey="Kunert, K J" sort="Kunert, K J" uniqKey="Kunert K" first="K. J." last="Kunert">K. 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Noctor</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire du Metabolisme (G.N.) and Laboratoire de Biologie Cellulaire (L.J.), Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire du Metabolisme (G.N.) and Laboratoire de Biologie Cellulaire (L.J.), Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Strohm, M" sort="Strohm, M" uniqKey="Strohm M" first="M." last="Strohm">M. Strohm</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name></author><author><name sortKey="Kunert, K J" sort="Kunert, K J" uniqKey="Kunert K" first="K. J." last="Kunert">K. J. Kunert</name></author><author><name sortKey="Foyer, C H" sort="Foyer, C H" uniqKey="Foyer C" first="C. H." last="Foyer">C. H. Foyer</name></author><author><name sortKey="Rennenberg, H" sort="Rennenberg, H" uniqKey="Rennenberg H" first="H." last="Rennenberg">H. Rennenberg</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Internode stem fragments of the poplar hybrid Populus tremula x Populus alba were transformed with a bacterial gene (gshl) for [gamma]-glutamylcysteine synthetase ([gamma]-ECS) targeted to the cytosol. Lines overexpressing [gamma]-ECS were identified by northern analysis, and the transformant with the highest enzyme activity was used to investigate the control of glutathione synthesis. Whereas foliar [gamma]-ECS activity was below the limit of detection in untransformed plants, activities of up to 8.7 nmol mg-1 protein min-1 were found in the transformant, in which the foliar contents of [gamma]-glutamylcysteine ([gamma]-EC) and glutathione were increased approximately 10- and 3-fold, respectively, without affecting either the reduction state of the glutathione pool or the foliar cysteine content. A supply of exogenous cysteine to leaf discs increased the glutathione content from both transformed and untransformed poplars, and caused the [gamma]-EC content of the transformant discs to increase still further. The following conclusions are drawn: (a) the native [gamma]-ECS of untransformed poplars exists in quantities that are limiting for foliar glutathione synthesis; (b) foliar glutathione synthesis in untransformed poplars is limited by cysteine availability; (c) in the transformant interactions between glutathione synthesis and cysteine synthesis operate to sustain the increased formation of [gamma]-EC and glutathione; and (d) the foliar glutathione content of the transformant is restricted by cysteine availability and by the activity of glutathione synthetase.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">12226433</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>112</Volume><Issue>3</Issue><PubDate><Year>1996</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Synthesis of Glutathione in Leaves of Transgenic Poplar Overexpressing [gamma]-Glutamylcysteine Synthetase.</ArticleTitle><Pagination><MedlinePgn>1071-1078</MedlinePgn></Pagination><Abstract><AbstractText>Internode stem fragments of the poplar hybrid Populus tremula x Populus alba were transformed with a bacterial gene (gshl) for [gamma]-glutamylcysteine synthetase ([gamma]-ECS) targeted to the cytosol. Lines overexpressing [gamma]-ECS were identified by northern analysis, and the transformant with the highest enzyme activity was used to investigate the control of glutathione synthesis. Whereas foliar [gamma]-ECS activity was below the limit of detection in untransformed plants, activities of up to 8.7 nmol mg-1 protein min-1 were found in the transformant, in which the foliar contents of [gamma]-glutamylcysteine ([gamma]-EC) and glutathione were increased approximately 10- and 3-fold, respectively, without affecting either the reduction state of the glutathione pool or the foliar cysteine content. A supply of exogenous cysteine to leaf discs increased the glutathione content from both transformed and untransformed poplars, and caused the [gamma]-EC content of the transformant discs to increase still further. The following conclusions are drawn: (a) the native [gamma]-ECS of untransformed poplars exists in quantities that are limiting for foliar glutathione synthesis; (b) foliar glutathione synthesis in untransformed poplars is limited by cysteine availability; (c) in the transformant interactions between glutathione synthesis and cysteine synthesis operate to sustain the increased formation of [gamma]-EC and glutathione; and (d) the foliar glutathione content of the transformant is restricted by cysteine availability and by the activity of glutathione synthetase.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Noctor</LastName><ForeName>G.</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratoire du Metabolisme (G.N.) and Laboratoire de Biologie Cellulaire (L.J.), Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Strohm</LastName><ForeName>M.</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Jouanin</LastName><ForeName>L.</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Kunert</LastName><ForeName>K. J.</ForeName><Initials>KJ</Initials></Author><Author ValidYN="Y"><LastName>Foyer</LastName><ForeName>C. H.</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Rennenberg</LastName><ForeName>H.</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>9</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12226433</ArticleId><ArticleId IdType="pii">112/3/1071</ArticleId><ArticleId IdType="pmc">PMC158033</ArticleId><ArticleId IdType="doi">10.1104/pp.112.3.1071</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 1995 Nov;109(3):1047-57</Citation><ArticleIdList><ArticleId IdType="pubmed">8552710</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Soc Trans. 1994 Nov;22(4):936-40</Citation><ArticleIdList><ArticleId IdType="pubmed">7698488</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1986 Jun 11;14(11):4393-400</Citation><ArticleIdList><ArticleId IdType="pubmed">2872655</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1992 Jun;99(2):428-33</Citation><ArticleIdList><ArticleId IdType="pubmed">16668902</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1984 Nov 26;12(22):8711-21</Citation><ArticleIdList><ArticleId IdType="pubmed">6095209</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Sep;106(1):233-239</Citation><ArticleIdList><ArticleId IdType="pubmed">12232324</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1988 Dec 9;16(23):11380</Citation><ArticleIdList><ArticleId IdType="pubmed">3205748</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochimie. 1986 Jan;68(1):121-32</Citation><ArticleIdList><ArticleId IdType="pubmed">3089305</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Versailles</li></settlement></list><tree><noCountry><name sortKey="Foyer, C H" sort="Foyer, C H" uniqKey="Foyer C" first="C. H." last="Foyer">C. H. Foyer</name><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L." last="Jouanin">L. Jouanin</name><name sortKey="Kunert, K J" sort="Kunert, K J" uniqKey="Kunert K" first="K. J." last="Kunert">K. J. Kunert</name><name sortKey="Rennenberg, H" sort="Rennenberg, H" uniqKey="Rennenberg H" first="H." last="Rennenberg">H. Rennenberg</name><name sortKey="Strohm, M" sort="Strohm, M" uniqKey="Strohm M" first="M." last="Strohm">M. Strohm</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Noctor, G" sort="Noctor, G" uniqKey="Noctor G" first="G." last="Noctor">G. Noctor</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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